Total RNA Isolation and Purification Protocols

• TRIZOL Total RNA Isolation Protocol
• Total RNA Isolation and Cleanup using Qiagen RNeasy with optional DNase treatment
• Total RNA Cleanup (45 - 100 ug) using Qiagen Rneasy
• Total RNA Cleanup (45 - 100 ug) with DNase Treatment
• Total RNA MinElute Cleanup (<45 ug) with DNase Treatment

Affymetrix Expression Protocols

Total RNA samples hybridized to Affymetrix GeneChips are prepared using the GeneChip 3' Amplification One-Cycle cDNA synthesis kit from Affymetrix.

• Total RNA to Double-stranded cDNA
• cDNA Cleanup
• In Vitro Transcription
• cRNA Cleanup
• cRNA Fragmentation
• Preparation of Hybridization Cocktail
• Expression Analysis Technical Manual

Illumina Expression Protocols

Total RNA samples hybridized to Illumina BeadChips are prepared using the Illumina TotalPrep RNA Amplification kit from Applied Biosystems/Ambion.

• Total RNA to Double-stranded cDNA
• cDNA Cleanup
• In Vitro Transcription
• cRNA Cleanup

Affymetrix Genotyping Protocols and Guidance

• 100k Genomic DNA Sample Preparation
• 100k Digestion
• 100k Ligation
• 100k PCR
• 100k Purification
• 100k Fragmentation and Labeling
• 100k Preparation of Hybridization Cocktail
• 100k Hybridization
• 100k Mapping Technical Manual (.pdf, 1.2 MB)

Applied Biosystems Protocols and Guidance

• Assays-on-Demand™ Gene Expression Products

• High-Capacity cDNA Archive Kit

• SYBR® Green PCR Master Mix and RT-PCR

• Primer Design for Allelic descrimination

• Assays-on-Demand™ SNP Genotyping Products

• Primer and Probe Design Tutorial

• Real-time/SNP ABI 7900HT Protocol

Other Information

• Keck Affymetrix Resource Description for Grant Write-Up
• Experimental Procedures for Grant Write-Up
• GCOS and DTT Software Tutorial