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W.M. Keck Facility
Yale University
300 George Street
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Keck
Home Page > Affymetrix GeneChip Service >
Real-Time PCR > Sample
Submission
GUIDELINES FOR SAMPLE SUBMISSION
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RT-PCR/SNP Sample Submission Form must be submitted along
with each sample.
Sample Preparation: We recommend that
users set up their own reactions in a 384-well plate. However, under certain circumstances we
may be
able to set up the complete reaction plate for an additional fee. The samples should be
supplied in a 384 well plate ready to be placed in the instrument. We will
be responsible for running the plate on the instrument and initial data
analysis. All of the sample information needs to be provided in a special
template format so that it can be directly imported into the SDS plate
document. TEMPLATES Please open these as Excel files. Substitute your
information in the fields on the template. Save the document as a text file and
then import it from the text file.
Plate set up: Plate set up will vary depending up on the
experiment. The following are general guidelines. For detailed protocols
or a consultation, please contact us.
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Each sample should be run in
duplicates at the minimum. Triplicates are preferred because if a single reaction is out of range,
it
can be eliminated from the analysis.
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NTC- No template control for each RNA/DNA.
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No RT control for each cDNA. No RT control
serves to determine if there is genomic DNA contamination in the RNA
preparation.
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