High protein concentration                                  Low protein concentration
Cell:               RNase           64 uM
Syringe:         2'-CMP        2.19 mM
2 uL injections

The area underneath each injection peak (top panel) is equal to the total heat released for that injection.  When this is plotted against the molar ratio of ligand added to macromolecule in the cell, a complete binding isotherm for the interaction is obtained (bottom panel).

The fitted isotherm provides the following binding parameters:

Data: RNAseEwa11_NDH
Model:   OneSites
Chi^2 = 9521.08

N =  0.9617   ±   0.001882            (Stoichiometry; mass ratio)
K  = 7.566   ±   0.1958  E5           (Binding constant)
delta  H =  -1.692 E4  ±   48.84     (Enthalpy of binding reaction)
delta  S  =   -28.90                        (Entropy of binding reaction)
 

Low protein concentration
 
 
 
 

RNase           11.4 uM 2'-CMP        0.544 mM	RNase           40 uM 2'-CMP        2.72 mM
Data: RNAseEwa112602_NDH Model:   OneSites Chi^2 = 22106.6 N =  0.8972   ±   0.00832  K  = 7.902   ±   0.369  E5  delta  H =  -1.783 E4  ±   226.8  delta  S  =   -31.83	Data: RNAseEwa112502_NDH Model:   OneSites Chi^2 = 8499.21 N =  1.006   ±   0.00352  K  = 6.806   ±   0.2378  E5  delta  H =  -1.655 E4  ±   92.44  delta  S  =   -27.91