biophysical resource Keck Facility at Yale

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Keck Foundation Biotechnology Resource Laboratory at Yale University

Surface Plasmon Resonance (SPR)

Biocore Biosensor

SPR is a universal tool for studying and characterizing macromolecular interaction in a label-free state. The system detects the binding reaction in real time by monitoring changes in mass concentration at the chip surface; the association and dissociation rate constants are determined directly from the reaction traces. Samples ranging from small molecules to crude extracts, lipid vesicles, viruses, bacteria and eukaryotic cells can be studied in real-time, without the use of labels and with little or no prior sample preparation.

The Biophysical Resource at Keck Facility offers the BiaCore instrument from BiaCore Inc. as an "open access" instrument that can be used by trained users. To schedule a training session please contact Ewa Folta-Stogniew

Excellent WebPages about SPR application that address various aspects of SPR application were created by Arnoud Marquart, so there is no excuse not to educate yourself. PLEASE read the SPR basics before scheduling a training session.

Read:
FAQ

Download and read the "HowTo" documents so you will have an idea about the general procedures

2007 BiaCore catalog lists currently available sensors chips. The Biophyscis resource provides the generic consumable like acetate buffers for pH-scouting, P20 detergent stock, or reagents for amine coupling. The sensor chips need to be ordered by intersted laboratory.

For SPR schedule go to SPR log_page

SPR performs the measurement in solution, but one of the interacting partners is immobilized on the chip surface; no modifications are needed but the measurement requires several consumable that have to be ordered form BiaCore.

Spectrofluorometer	SPR BiaCore	ITC	Stopped-Flow Spectrofluorometer	Fluorescence Polarization System	Light Scattering Service	Biophysical resource Front Page

Comments or suggestions for improving this Web site or the service? E-mail me (Ewa Folta-Stogniew)

Last Modified 2006 by Ewa Folta-Stogniew