|
Description |
|
|
Limit of detection of differential protein expression |
1.3 fold above background |
|
Dye linkage chemistry |
N-Hydroxy-succinimide (NHS) linkage to primary amines |
|
Approximate MW of CyDye DIGE fluors |
580 Da |
|
Labeling stoichiometry |
On average, 3% of protein will have one dye molecule attached |
|
Limit of detection for quantifying protein ratios |
Similar to silver staining, from 0.25 - 0.95 ng or 5 -19
fmol of a 50 kD protein1,
4 |
|
Dynamic range |
104 as compared to only about 101 for
CB of silver staining1 |
|
Maximum number of spots quantified/gel with high protein load |
>1,0502 |
|
Recommended amount of protein |
50 ug/sample |
|
Recommended concentration of protein extract |
5-10 mg/ml |
|
Approximate supply cost/gel assuming 3 dye labeling and post-staining of
26 x 20 cm gels with SYPRO Ruby |
$150/gel |
