Primer Sequences

Outlined below are the primer sequences that we have used through the years in our STANDARD reactions. You may want or need to have these custom primers synthesized. They may differ a little from proprietary primers. That does not mean that primers purchased from conventional vendors will not work - we have simply found that this set of primers works well when sequencing with a wide array of vectors. If you wish to explore the custom primers option, we have some primer design tools listed on our Primer Guidelines page.

Primer	Sequence (5'-3')
M13-21	TGT AAA ACG ACG GCC AGT
M13-47	CGC CAG GGT TTT CCC AGT CAC GAC
M13-REV4	TCA CAC AGG AAA CAG CTA TGA C
T7	TAA TAC GAC TCA CTA TAG GG
T7TERM	GCT AGT TAT TGC TCA GCG G
T3	ATT AAC CCT CAC TAA AGG GA
PCRIIT7	CGA CTC ACT ATA GGG CGA ATT GGG
PCRIISP6	GGT GAC ACT ATA GAA TAC TCA AGC
SP6	GAT TTA GGT GAC ACT ATA G
poly TG	(T)₂₅G
poly TC	(T)₂₅C
poly TA	(T)₂₅A
SK	TCT AGA ACT AGT GGA TC
KS	CGA GGT CGA CGG TAT CG

Primers may be ordered via the Keck Biotechnology Resource Lab.