DNA/RNA QC Analysis Service
Cost: Please see https://medicine.yale.edu/keck/dna/fees/
Instrument: Agilent TapeStation 2200
The Agilent Tapestation allows researchers to analyze the quantity and size of DNA or RNA while using only a few microliters of precious sample.
We currently offer 3 DNA kits and 1 RNA kit:
- Genomic DNA assay
- High Sensitivity DNA 1000 assay
- High Sensitivity DNA 5000 assay
- High Sensitivity RNA assay
Please see below for assay limits and specifications. If you need assistance choosing the proper assay, please feel free to contact us.
Standard Turnaround Time
Standard turnaround time is 1 business day. Yale users will receive priority over non-Yale users. If you anticipate usage, please email the lab at dnaseq@yale.edu to determine kit availability; especially if you are submitting a large batch.
Submission Requirements
- ≥2 µL of sample in a clearly labeled tube; any size/type is accepted.If submitting RNA samples, please hand deliver and have samples on wet ice.
*Be sure your sample buffer contains no more than 7 mM KCl, 20 mM Phosphate Buffer, 20 mM Guanidine-HCl, 80 mM NaCl, or 20 mM Acetate as high salt concentrations will cause electrophoresis failure
**We will not be responsible for electrophoresis failures due to improperly quantitated samples or samples in buffer with high salt concentrations.
Specifications
For DNA
| Assay Type | Description | Sizing Range (bp) | Quantitative Range (ng/uL) | Notes |
|---|---|---|---|---|
| DNA Assays< | Genomic DNA< | 200-60000< | 10-100< | *provides DIN< |
| High Sensitivity D1000< | 35-1000< | 0.01-1< | ||
| High Sensitivity D5000< | 100-5000< | 0.01-1< | ||
| RNA Assay< | RNA< | 100-6000< | 1-10< | *provides RIN< |
Data Return
Once the analysis is complete, we will email you a report that includes the gel image, electropherogram & Peak Table. We also include the raw data file which you can view on a PC using Agilent’s TapeStation Analysis Software.
Turnaround time for this service is approximately 24 hours.
Agilent Technologies TapeStation 2200
Developed by the company behind the industry-leading 2100 Bioanalyzer, the Agilent 2200 TapeStation system offers scalable throughput and rapid results, making it an ideal solution for quality control of biological samples in next-generation sequencing, microarray and qPCR workflows. The TapeStation is a revolutionary tape-based platform for simpler, faster and more reliable electrophoresis. It uses credit card-sized, disposable Agilent ScreenTape that is available for DNA and RNA applications, and low sample volumes, only 2µl of precious sample per run.