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DNA Microarray
Resource > Custom Arrays
CUSTOM ARRAYS
An increasing number of investigators are interested in creating custom
arrays of PCR products and oligonucleotides as microarray technology
matures and expands into new avenues of research. A number of reasons
for creating custom arrays are listed below:
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To create a subset of probes of interest as a result of initial
screening or expression studies using "genome-wide" arrays.
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To create an array of probes for non-standard applications of
microarray technology for assay development.
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To create an array of probes on a genome scale for specific organisms,
tissues, subsets of cells that may or may not be represented by
"generic arrays".
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To create a non-standard format of probes e.g. unusual slide surface,
attachment, subarray pattern etc. for specific applications.
The Microarray Resource is able to work closely with individual
investigators, Yale and Non-Yale, on a cost-recovery or collaborative
basis, to fabricate custom arrays. The Resource staff provide guidance
and suggestions on array design based on their five years of
experience.
Standard procedure for Custom Microarray
irina.tikhonova@yale.edu)
via e-mail with brief outline of project.
Schedule a meeting to discuss requirements and time frame in detail.
Issue formal
*quotation for
project.
Acceptance and implementation.
*A formal
cost-recovery quotation outlining the scope of work and
responsibilities of the Resource and participating lab is issued after
an initial consultation with the Resource staff. Test prints are usually
necessary when developing a custom array - especially where the scope
of the project is far removed from the standard operating procedures of
the Resource. The test prints are charged on a cost-recovery basis.
Once a project has been formulated and printing begins, ONLY
Resource staff are responsible for
implementing the arrays at the level of instrument operation within the
lab and use of clean rooms for printing and slide processing.
(The Resource reserves
the ability to add additional charges, after discussions, if the
project extends beyond the confines of the original scope of work).
Because "custom arrays" are by definition
variable in their requirements it is somewhat difficult to estimate
costs at the start of a project. In general the current rough estimate
for each slide (minimum 50 slide per run) is approximately $75 (Yale
cost - including PLL slide) for a 48 pin print on Omnigrid 100 with
Server Arm. All test prints, robotic transfers, plasmid preps, PCR, gel
characterization, non-standard slides, print plates are recovered as
separate supplemental charges outlined in the initial quotation.
Please contact
Irina Tikhonova
via e-mail or
call (203) 785-5209 with questions.
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