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Keck
Home Page > DNA Microarray
Resource > Goals
MICROARRAY RESOURCE GOALS
The overall goal of the
Resource, located
in 300 George Street,
2nd Floor, Suite 2110, is to bring DNA microarray technology
within reach of Yale investigators by establishing a cost
recovery unit whose instruments are operated by its staff
members.
Services offered
include:
- array on to microscope
slides standardized human, mouse, rat and Arabidopsis cDNAs
and oligonucleotides from Resource collections obtained
from both commercial and academic sources.
- provide full custom array services for cDNA and
oligo arrays.
- provide labeling services to link monofunctional
cy-dyes to aminoallyl-labeled first strand cDNA probes
generated by researchers from custom RNA prepared
according to guideline protocols provided by the
Resource
- hybridize custom
fluorescently labeled probes (cy-3 and cy-5 etc) - prepared
by each researcher according to guideline protocols
provided by the Resource
- scan the resulting
slides and acquire image and numerical data for each
fluorescent wavelength
- analyze the resulting
data to generate cy-3:cy-5 ratios, scatter plots, cluster
analyses etc.
The goal is to provide an
avenue for researchers to use any or all of
these services as they require, with the option of
performing others in their own lab.
Currently we have two
Genomic Solutions-Gene
Machines Omnigrid
robotic arrayers
with
Telechem
Stealth split pins
housed in state-of-the art environmentally controlled Class100
cleanrooms. We print mainly on Corning ULTRA-GAP slides and are using multipurpose
hybridization chambers from
Gene
Machines as
well as an ArrayBooster auto-hyb station from
Advalytix.
The resource has a
PE
ScanArray
5000XL
scanner (16
fluor capability) with
QuantArray
software to analyze
arrays and a Cy3/Cy5 dedicated
Axon
GenePix 4000A and
4200
scanner and
GenePix
Pro3.0 and 5.0 software.
For Resonance Light Scattering (RLS) applications rather than
fluorescence we have a
HiLight Reader.
Yale has created a web
accessible database, the Yale Micorarray Database. This is Oracle based and is a queriable
repository for .tiff images and data files. At this time, YMD links to various downstream software for data
analysis and visualization. Further details at the
Yale Micorarray Database.
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