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Keck
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DNA Sequencing
> Sequencing Protocols >
High Volume
High Volume
Plate Submission Policies and Procedures
High Volume Submission Policies
High Volume Plate Submission
Protocol
Standard Turn around time
High
Volume Submission Policies
1.) The high volume, 96
well plate format is available for researchers who are experienced users of the
facility and are confident in their methodology for template and primer
preparation. We sequence and charge for all samples submitted on the plate
regardless of the results, unless it is a clear technical error on our part.
2.) All high volume plate
submissions must be in red 96-well Eppendorf twin tec plates or Applied
Biosystems Optical 96-well reaction plates. The Eppendorf twin tec plates
are located in the Yale stockroom.
Ordering
Information for Red Eppendorf twin tec 96-well plate through
Brinkmann by phone at 1-800-645-3050:
Red Eppendorf twin tec
96-well plate: Part# 951020389
Strip caps for Eppendorf
96-well PCR plate: Part# 951023035
Ordering Information for
Applied Biosystems Optical
96-Well Reaction Plates by phone at 1-800-327-3002
Applied Biosystems Optical 96-Well Reaction
Plates: Part# N801-0560
Strip Caps for ABI 96-Well Reaction Plates:
Part# N801-0534
3.) The minimum number of samples for
a single high volume plate is 48. When submitting less than 96 samples on a plate, please submit in
multiples of 12- placing the samples in rows of 12 consecutively i.e.
begin at A1 through A12 then B1-B12, C1-12, etc.
4.) High volume plates should be
dropped off with a high volume submission sheet ( Yale,
Non-Yale)
5) Our charge for requested repeats
on a high volume plate is $6 a repeat. If the repeated sequence looks
better than the initial sequence you will not be charged. a
High Volume Plate Submission
Protocol
High Volume
96-well reaction
Add to each well:
16
µl containing
600 ng plasmid
DNA
or
16
µl
containing 10-20 ng
per 200bp of PCR product
(e.g.
for a 400bp PCR product each well should contain 20-40 ng DNA)
or
16
µl containing
2000 ng
cosmid or
lambda
DNA
or
16
µl containing
4000 ng BAC
DNA
Add to each
well:
2
µl of
4 µM custom or
universal primer
Total Volume: 18
µl per well
Cap the plate firmly
using the strip caps and wrap each plate with
parafilm.
Be sure to label each
plate with a unique name. Results will be labeled with the
researchers code initials-plate name -and well-coordinate (RES
CODE-plate name-A1-12, RES CODE-plate name-B1-12 etc). The researcher
is responsible for holding the "key" to the well co-ordinates for
sample identification.
Standard Turn around time
Our turn around time for a single plate is 24-48 hours from the time we
receive your plate (except for plates received on Friday). Please allow
extra time when you submit more than one plate at a time.
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