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Keck
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What's New?
What's New?
March 1, 2008:
We have reduced prices for high volume plates. Please visit our price schedules
under services charges, to view how the new price per well applies to your plate
format.
October 1, 2007:
We are adding a new drop off location for
sequencing samples, Amistad room 420. We will have a courier pick up from
this location two times daily, at approximately 9am and 3pm.
March 27, 2006:
We are starting a new
Ready to Run (RTR) sequencing service on a trial basis: we will accept
cleaned-up sequencing samples that are ready to load onto the ABI 3730xl. They
MUST be in ABI MicroAmp Optical plates, cycle sequenced with ABI BigDye
Terminator v. 3.1, and cleaned up to remove excess dye and other contaminants
(e-mail
nancy.dasilva@yale.edu
for protocols). The charge for RTR is $144/96 well plate ($1.50/sample) and you
will be charged regardless of the results unless we observe an obvious sequencer
problem.
March 1, 2006:
Labeling premixed tubes with barcodes has
allowed us to process more samples with less staff involvement. As a
result, we have reduced our prices for premixed sequencing samples submitted in
individual tubes from $12 to $10 a sample. Also,
templates that have a high GC content (>70%),
known structures, or BAC that require our difficult template protocol will cost an extra $2.
These samples have to be sequenced separately by hand and require different
reagents. Please contact us
for more information.
DNA Sequencing Drop-off Hours and
Locations
Medical School Area: BCMM
108, TAC S352, AMISB 420:
- Drop-off
hours: 8:30 am-4:30pm
- Estimated pick-up times: 9:00
am and 3:00 pm
- Samples submitted after 3:00 pm will be
picked-up the next morning and processed on that day. Samples submitted
after the 9:00am pickup will be processed the next business day.
Science Hill Area: JWG
304 & OML 114:
- Estimated pick-up times: 9:00
am and 2:30 pm
- Since the MB&B pickup is an unsecured area
and the courier service is involved, we cannot be responsible for samples that
do not reach us from these locations.
300 George St Room 2127:
This site is available for questions
between the hours of 1 and 3pm weekdays only.
- Drop-off hours:
8:30am-5:00pm
High Volume Sequencing: 96-well Plate
Format
The high volume, 96-well plate format is
available for researchers who are
experienced users of the facility who are confident in their
methodology for template and primer preparation. We sequence and charge for the whole plate
regardless of the results, unless it is a clear error on our
part.
Please check the link high volume sequencing:
96-well plate
Turnaround Time
- Our OPTIMUM
turnaround time is
1 day from the day of
submission.
- For example: 4
samples submitted on Monday morning will be ready by Tuesday lunchtime; 30
samples submitted on Monday, 10 will be ready on Tuesday and then 10 on each
subsequent day until complete.
- When we take in a
particularly large number of samples (e.g. 400 or more) in one day, this
significantly changes our turnaround time.
It may increase to as much as 2 days.
-
Primer walking projects are also subject to
turnaround time increases when we have a large number of submissions within a
short period of time.
Data Return
We now have 100% of our customers
successfully using the ftp-server. We welcome your comments via
e-mail.
- This requires that each lab have access
to the Yale ftp server at ftp.med.yale.edu.
- Access is possible using
ftp-client software
such as Fetch for
Macintosh and FTP-Voyager
for IBM/PC's.
- WE NOW HAVE access via our
WEB-site for data
return via FTP at host ftp.med.yale.edu which makes access very
simple. The link can be found in the Site Index. Simply enter you
user ID and password to enter your lab account and obtain
data.
For new users of our facility : ftp-accounts
are created after
we receive your first submission. Account information
is returned to the researcher via e-mail before the first data are
available.
Data Removal from FTP
accounts
It is critical that you back-up data from the
server as soon as it arrives, so that you will not lose valuable data when it is
deleted. Typically, data is left on the server for 4-6 weeks.
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