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Important Notices
Important Notices
March 1, 2008:
We have reduced prices for high volume plates. Please visit our price schedules
under services charges, to view how the new price per well applies to your plate
format.
October 1, 2007:
We are adding a new drop off location for
sequencing samples, Amistad room 420. We will have a courier pick up from
this location two times daily, at approximately 9am and 3pm.
March 27, 2006:
We are starting a new
Ready to Run (RTR) sequencing service on a trial basis: we will accept
cleaned-up sequencing samples that are ready to load onto the ABI 3730xl. They
MUST be in ABI MicroAmp Optical plates, cycle sequenced with ABI BigDye
Terminator v. 3.1, and cleaned up to remove excess dye and other contaminants
(e-mail
nancy.henriques@yale.edu
for protocols). The charge for RTR is $144/96 well plate ($1.50/sample) and you
will be charged regardless of the results unless we observe an obvious sequencer
problem.
March 1, 2006:
Labeling premixed tubes with barcodes has
allowed us to process more samples with less staff involvement. As a
result, we have reduced our prices for premixed sequencing samples submitted in
individual tubes from $12 to $10 a sample. Also,
templates that have a high GC content (>70%),
known structures, or BAC that require our difficult template protocol will cost an extra $2.
These samples have to be sequenced separately by hand and require different
reagents. Please contact us
for more information.
We have a new repeat policy
for samples on high volume plates please see High Volume
Plate Policies.
Since January 1, 2002,
the printing of four-color plots has been discontinued. Instead we will
distribute to all users via our FTP site the ABI data file in either Macintosh
or PC/Unix format. By using the appropriate freeware/shareware or purchased software, the electropherogram may be viewed, the sequence edited, and if desired printed to a
color printer.
The program for the Macintosh
OS9 or lower
is EditView and is available at:
http://www.appliedbiosystems.com/support/software/dnaseq/installs.cfm
The program for Macintosh OS10 is 4
Peaks and is available at:
http://www.mekentosj.com/4peaks/index.html
Options for PC is available at:
http://www.technelysium.com.au/chromas.html
Sequence Scanner Software v1.0
Both programs download as
self-extracting files--download them to your computer and double click on
the icon to start the extraction process.
More information and freeware is
available on our Bioinformatics page.
For your convenience, a self-service sample submission areas
are located at: BCMM 108, TAC S352, AMISB 420, JWG 304,
and OML 114.
No one is available any of
these
sites to answer questions. All in person inquiries must be made at the 300 George
St. address, between the hours of 1 to 3 pm Monday through Friday.
If you have any questions and
cannot make these times, please contact us at 7-2566 to schedule an
appointment. Alternatively, email messages may be sent to
dnasequencing@yale.edu.
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