Illumina

MicroRNA Expression Profiling: 12 sample arrays

Illumina has developed a powerful method for profiling the expression levels of a wide range of miRNA based on our proven DASL Assay.  Human and Mouse MicroRNA Panels measure the levels of 735 or 380 miRNA, respectively.  These panels contain high-value content, targeting miRNA described in the miRBase database and the literature.

microRNA expression profiling panel highlights

• high-value coverage - target miRNA described in miRBase and the literature
• cost effective - industry’s lowest pricing per sample
  
• low sample input - 100-200 ng of total RNA from cultured cells, fresh frozen, or FFPE tissue
  
• high specificity - single-nucleotide discrimination between closely related miRNA species
  
• flexible format - 12-sample Universal BeadChips or 96-sample Universal Arrays fit any study size

assay technology: dasl assay

The cDNA-mediated Annealing, Selection, Extension, and Ligation (DASL) Assay allows for RNA profiling of up to 1,536 targets using partially degraded RNA as found in formalin-fixed, paraffin-embedded (FFPE) samples. Using either the Universal-16 or the Universal-96 choice Array Matrix format for sample processing, the DASL Assay offers researchers the opportunity to analyze hundreds to thousands of RNA transcripts derived from previously collected, preserved samples. The DASL Assay leverages the proven GoldenGate Assay protocol to provide high-quality data for an RNA-based application. Because the probe groups span only about 50 bases, partially degraded RNA can be used in the assay. The availability of additional, unique probe sequences per gene increases the sensitivity of the assay, allowing the quantitation of low-abundance transcripts, even in partially degraded samples.

dasl assay highlights

• Reproducible RNA profiling: Profile partially-degraded RNAs such as those found in formalin-fixed, paraffin-embedded (FFPE) samples
  
• Proven sensitivity: Detect low-abundance transcripts
  
• Ideal for clinical sample types: Requires an input of just 100–200 ng total RNA
  
• Flexible formats: Process in groups of 24 or 96 samples
  
• Scalable solution: Automatable platform with low per-sample pricing