NimbleGen Methylation analysis finds methylated regions genome-wide or within targeted promoters or CpG islands with NimbleGen's high-resolution DNA methylation analysis technology.

DNA methylation microarray service consists of the following steps:

• Customer selects catalog array design, or works with Roche NimbleGen bioinformatics scientists to design a custom array. Customers purchase catalog arrays or custom arrays from NimbleGen.
• Customer prepares samples, including immunoprecipitation with anti-5-methyl-cytidine (MeDIP, recommended) or the MBD protein method. The DNA is amplified (if required) according to recommended protocols. DNA needs to be 200 bp or greater in size.

• Keck labels the samples, performs the hybridization, scans the array, extracts the data, and performs a preliminary data analysis.
• The customer receives the raw data, scaled log2-ratio data, p-value data, peaks data (methylated regions), genome annotation, reports listing peaks with associated transcripts and gene annotation information.