Sample Submission

GA Sample Submission Form

If you would like Keck to prepare samples for sequencing on Illumina’s Genome Analyzer, then please see appropriate sample submission requirements.

If you would prefer to prepare ready-to-sequence libraries on your own, please contact Illumina Sales at (800)-809-4566 or Ken Adams at (978)-448-2341 for pricing and ordering information.

If you prepare your own libraries and would like only cluster generation and incorporation services, at least 10ul of 10nM library suspended in EB Buffer should be submitted.

For pricing, please refer to Services.

Genomic DNA

1 – 5 ug of Purified DNA (5ug recommended) suspended in TE Buffer in a volume not to exceed 50ul.  DNA should be as intact as possible, with an OD260/280 ratio of 1.8-2.

Sequence-Capture (Seq-Cap)

Whole Transcriptome (mRNA-Seq) Profiling

1 - 10ug of Purified RNA suspended in nuclease-free H2O in a volume less than, or equal to, 50ul. RNA should be as intact as possible, with OD260/280 ratio of 1.8-2 and an OD260/230 ratio of 1.8-2.  RNA integrity should be verified via an Agilent Technologies 2100 Bioanalyzer or 1% agarose gel.   High-quality RNA will show a 28s rRNA band twice the intensity of the 18S rRNA band.

Small RNA Querying

1 - 10ug of Purified RNA suspended in nuclease-free H2O in 10ul volume.  RNA should be isolated using a technique that preserves small RNAs (using Qiagen’s miRNeasy kit, for example). RNA should be as intact as possible, with OD260/280 ratio of 1.8-2 and an OD260/230 ratio of 1.8-2.  RNA integrity should be verified via an Agilent Technologies 2100 Bioanalyzer or 1% agarose gel.   High-quality RNA will show a 28s rRNA band twice the intensity of the 18S rRNA band.

ChIP-Enriched DNA Sequencing

10ng of purified, ChIP-enriched, qPCR-verified DNA suspended in 30ul TE Buffer should be submitted.

Recommended # of Lanes Assuming 30x coverage (=99.95% accuracy) for Human Genome

Assay	Read Length	# of Flowcells or Lanes
Genomic DNA	2 x 75 bp	5-6 flowcells.
mRNA-Seq	1 x 50 bp	3 lanes.
Small RNA	1 x 35 bp	One lane.
ChIP-Seq (assuming 1% human genome capture)	1 x 35 bp	One lane.
Seq-Cap (Exome)	2 x 75 bp	Two lanes.