|
Keck
Home Page >
Oligo Synthesis >
UltraMild Synthesis
UltraMild Synthesis
Some modifications require the
use of UltraMild columns, phosphoramidites and reagents that allow for mild
cleavage and deprotection conditions. Some of these modifications include:
- cyanine dyes including Cy3,
Cy3.5, Cy5 and Cy5.5
- TAMRA-dT and 3'-TAMRA
- acridine
- etheno-dA
- 5,6-Dihydro-dU
- 5'-iodo-dT
- 5'-OH-dU
- 5'-OH-dC
- thymidine glycol
The price of these UltraMild
columns, phosphoramidites and reagents along with the price of any modified
phosphoramidites will be added to the normal oligo price. In most cases, oligos
requiring UltraMild reagents are cleaved and deprotected using 0.05 M potassium
carbonate in MeOH. The cleaved and deprotected oligo will be delivered wet in 3
ml of 0.025 M potassium carbonate containing 50% MeOH and 1.0 M TEAA. Since the
potassium salts are not volatile, the oligos will need to be desalted before
they are used. (Note: If reverse-phase chromatography will be used for
desalting, the MeOH content will need to be lowered to about 5% by diluting or
evaporating.)
Please see our Modifier Pricing Page for
UltraMild reagent prices.
|
