|
GENOMIC
SERVICES
|
|
Microarrays |
Yale ($)
|
Non-Yale
($)
|
|
Non-Profit
|
|
GENE EXPRESSION (array cost not included) |
Total RNA Q.C. only (nanodrop & bioanalyzer)
|
$15 |
$17 |
AFFYMETRIX
|
Expression Full Service
|
$300 |
$345 |
2-cycle Full Service
|
$500 |
$575 |
Exon Full Service
|
$450 |
$518 |
Gene Array Full Service
|
$390 |
$449 |
Prelabeled cRNA Hyb Only
|
$175 |
$201 |
EXIQON
|
miRNA Expression Profiling Full Service
|
$210 |
$230 |
ILLUMINA
|
Expression Full Service Per Sample
|
$140 |
$161 |
Prelabeled cRNA Hyb Only Per Chip
|
$80 |
$92 |
Expression DASL Assay, 96 samples
|
$1200 |
$1380 |
NIMBLEGEN
|
Expression Full Service
|
$400 |
$440 |
Expresssion Hyb Only
|
$145 |
$160 |
SEQUENOM
|
Expression Full Service (pricing based on
the size of project)
|
Please Inquire |
SPOTTED
|
Spotted Catalog Arrays (see list)
|
$100 - $150 |
Expression Full Service
|
$240 |
$265 |
Expresssion Hyb Only
|
$125 |
$135 |
Scan Only
|
$25 |
$30 |
Raw Data Analysis using GenPix software
|
$97 / hr |
$112 / hr |
Custom Microarray (pricing based on the size
of project)
|
Please Inquire |
|
SOLEXA SEQUENCING (Illumina Genome Analyzer) |
Cluster Generation + sequencing (includes
reagents and labor)
|
$6335 |
$7285 |
Library preparation
|
Please Inquire |
|
GENOTYPING (array cost not included) |
Genomic DNA Q.C. only (spectrophotometer &
agarose gel)
|
$15 |
$18 |
AFFYMETRIX
|
Genotyping Full Service
|
Please Inquire |
Genotyping Hyb Only
|
$70 |
$81 |
ILLUMINA
|
Genotyping Full Service Per Sample
|
$100 |
$115 |
Golden Gate Full Service Per 96 Samples
|
$1200 |
$1380 |
NIMBLEGEN
|
Genotyping Full Service
|
$275 |
$305 |
Genotyping Hyb Only
|
$145 |
$160 |
SEQUENOM
|
Geontyping Full Service (pricing based on
the size of project)
|
Please Inquire |
|
INSTRUMENT USAGE |
ABI 7900HT Realtime PCR Plate Run (equipment
use only)
|
$60 |
$69 |
ABI 7900HT SNP Plate Run (including real
time run and post read)
|
$80 |
$92 |
ABI 7900HT SNP Plate Post Read Only
|
$20 |
$23 |
|
DATA ANALYSIS: EXPRESSION & GENOTYPING |
Please refer to the Keck Biostatistics
Department by contacting Amy by e-mail.
|
$97 / hr |
$112 / hr |
BeadStudio, GCOS, GenePix, GeneSpring,
NimbleScan, SDS Workstations
|
Free |
GCOS or BeadStudio Software Tutorial (30
minutes)
|
Free |
|
|
|
| DNA
Sequencing |
Yale ($)
|
Non-Yale
($)
|
|
Non-Profit
|
Individual Tubes Premixed Reactions
|
4.50 |
8 |
Individual Tubes Premixed, Wrong Tubes
|
6.50 |
10 |
High Volume 96 well sequencing
|
48 - 79 samples per plate
|
3.50 /
well |
5.25 /
well |
80 - 96 samples per plate
|
2.70 /
well |
4.25 /
well |
96-well plate/PCR purification &
sequencing
|
3.20 /
well |
N/A |
384-well plate/premix
|
2.30 /
well |
N/A |
384-well plate/PCR purification &
sequencing
|
2.50 /
well |
N/A |
Primer Walking
|
Sequence runs each
|
27 |
30.50 |
Primers as needed for primer
walking
|
$0.60 per
base + $2.85 set up per primer |
Consensus Sequence Assembly
and Editing
|
25 / kb |
25 / kb |
Difficult templates
(includes BAC, 70% GC rich, hairpin structures that require altered
sequencing methods)
|
$2 extra charge |
$2 extra charge |
|
|
|
| Oligonucleotide
Synthesis |
Yale ($)
|
Non-Yale
($)
|
|
Non-Profit
|
|
Consulting Charge (per hour) |
97 |
112 |
|
Unpurified DNA |
40 nanomole scale (1-60 bases,
unmodified)
|
Setup charge
|
2.00 |
2.30 |
Residue charge
|
0.37 |
0.43 |
40 nanomole scale (>60 bases with
A,G,C or T @ 3' end)
|
Setup charge
|
3.50 |
4.00 |
Residue charge
|
0.50 |
0.58 |
0.2 micromole scale (A,G,C or T @ 3' end,
LV200 nanomole)
|
Setup charge
|
4.00 |
4.60 |
Residue charge
|
0.95 |
1.09 |
0.2 micromole scale (non-standard
3' end)
|
Setup charge
|
8.00 |
9.20 |
Residue charge
|
1.25 |
1.44 |
1.0 Micromole Scale
|
Setup charge
|
5.00 |
5.75 |
Residue Charge
|
1.45 |
1.67 |
|
Purified DNA |
Cartridge Purified/Desalted DNA-all
synthesis scales
|
Surcharge per oligo
|
25.00 |
28.75 |
Gel purify from 40 nanomole
scale
|
Surcharge per oligo
|
45.00 |
51.75 |
Gel purify from 0.2 micromole (LV200
nanomole)
|
Surcharge per oligo
|
135.00 |
155.25 |
Gel purify from 1.0 micromole scale
|
Surcharge per oligo
|
405.00 |
465.75 |
RNA/2'-O-Methyl RNA
|
1.0 Micromole Scale
|
Setup charge
|
18.00 |
20.70 |
Residue charge
|
8.50 |
9.78 |
|
|
|
|
|
PROTEOMIC
SERVICES
|
|
| Amino
Acid Analysis |
Yale ($)
|
Non-Yale
($)
|
|
Non-Profit
(effective 4/1/05)
|
Hydrolysis
|
15 |
20 |
Oxidation
|
25 |
30 |
Analysis
|
75 |
83 |
Tryptophan/MSA Hydrolysis
|
40 |
45 |
|
|
|
| Biophysics |
Yale ($)
|
Non-Yale
($)
|
|
Non-Profit
|
HPLC SEC/Laser Light Scattering
(Add Set-up Surcharge below)
|
195/sample |
215/sample |
Standard Running Buffer
Set-up Surcharge
|
300
/sample set |
320/sample set |
Customer Requested Running
Buffer Set-up Surcharge
|
350
/sample set |
375/sample set |
SpectraMAX Spectrofluorometer
|
30 / hour
|
40 / hour
|
Stopped-Flow Spectrofluorometer
|
Working
group: $22/hour
Non-working group: $60/hour |
$80/hour |
Stopped-Flow Spectrofluorometer Training
|
230 |
250 |
Open Access Dynamic Light
Scattering Instrument
|
100 / hour
|
115 / hour
|
Open Access DLS Training
|
230 |
250 |
Isothermal Calorimetry (ITC)
|
15 / hour |
18 / hour |
ITC 2-day training course
|
250 |
270 |
SPR Service
|
220 / day |
250 / day |
|
|
| Peptide
Synthesis |
Yale ($)
|
Non-Yale
($)
|
|
Non-Profit
|
| Peptide Reverse Phase HPLC (>10
mg scale) |
|
|
set-up charge
|
140 + |
161 + |
per peak charge
|
140 |
161 |
|
|
|
| Peptide
Synthesis/Large Scale (0.5 mmol) |
Yale ($)
|
Non-Yale ($)
|
|
Non-Profit
|
Residues 1-20
|
61 / Res |
67 / Res |
Residues 21-51
|
91 / Res |
100 / Res |
Residues 51 +
|
121 / Res |
133 / Res |
Amino-terminal Acetylation
|
61 |
67 |
His/Trp/Cys
|
+61 / Res |
+67 / Res |
Cleavage/Purification/MS/AAA
2
|
545 |
600 |
Resin Split
|
121 |
133 |
|
|
|
| Peptide
Synthesis/Small Scale (25 - 100 µmol) |
Yale ($)
|
Non-Yale
($)
|
|
Non-Profit
|
25 µmole Scale
|
Crude, 5-25 residues, 5-50
mg
|
220 |
242 |
Purified, 5-25 residues,
1-10 mg
|
495 |
545 |
50 µmole Scale
|
Crude, 5-25 residues, 10-100
mg
|
330 |
363 |
Purified, 5-25 residues,
2-20 mg
|
605 |
666 |
100 µmole Scale
|
Crude, 5-25 residues, 20-200
mg
|
550 |
605 |
Purified, 5-25 residues,
4-40 mg
|
825 |
908 |
|
|
|
| Amino
Acid Sequencing |
Yale ($)
|
Non-Yale
($)
|
|
Non-Profit
(effective 4/1/05)
|
Residues 1-5
|
325 |
370 |
Residues 6 - End
|
48 / Res |
55 / Res |
