Parameter
|
<50 pmol
|
51-100 pmol
|
101-200 pmol
|
>200 pmol
|
Total
|
| |
|
|
|
|
|
Number of proteins digested |
35 |
76 |
69 |
55 |
235 |
Average mass of protein (kD)
|
87 |
59 |
59 |
55 |
62 |
Average amount of protein
digested (pmol) |
32 |
75 |
139 |
328 |
147 |
Median amount of protein
digested |
29 |
76 |
134 |
282 |
112 |
Average gel volume |
33 |
38 |
66 |
99 |
|
Average density of protein
band (µg/mm3) |
0.100 |
0.216 |
0.248 |
0.498 |
0.274 |
Average amount of protease
added (µg) |
0.966 |
1.102 |
1.553 |
2.377 |
|
Total number of peptides
sequenced |
75 |
156 |
154 |
106 |
491 |
Average number of peptides
sequenced/protein |
2.1 |
2.1 |
2.2 |
1.9 |
2.1 |
%Peptides sequenced that
provided >6 positive residues |
80.0 |
80.1 |
82.5 |
84.9 |
81.9 |
Average % initial yield |
16.2 |
11.2 |
11.0 |
12.0 |
12.1 |
Median % initial yield |
10.4 |
7.3 |
7.0 |
9.2 |
7.30 |
%Peptides that provide no
sequence |
10.7 |
8.3 |
7.1 |
5.7 |
7.74 |
%Peptides that are mixtures |
9.3 |
11.5 |
9.7 |
9.4 |
10.18 |
Average number residues
called/peptide sequenced |
11.5 |
11.1 |
12.8 |
13.9 |
12.3 |
Overall average background
(µg) |
- |
- |
- |
- |
0.08 |
| |
|
|
|
|
|
Overall digest success rate |
94.3 |
97.4 |
97.1 |
98.2 |
96.8 |
Overall %Known Proteins |
89.7 |
67.2 |
64.6 |
81.6 |
73.2 |
